백서에서 혈관냉동 시간에 따른 동맥의 혈관경축 해소 효과
THE EFFECTS OF VASCULAR FREEZING TIME ON ARTERIAL VASOSPASM IN RATS
소속 상세정보
오희균/Hee-Kyun Oh
KMID : 0355619980240030290
Abstract
Ⅴ. 결 론
동맥의 혈관경축 해소를 위한 목적으로 혈관냉동을 시행시 냉동시간에 따른 혈관경축 해
소 효과와 혈관에 미치는 영향을 비교 검토함으로서 효과적이고 적절한 혈관냉동 시간을 알
아보고자 본 연구를 시행하였다. 백서의 대퇴동맥에 각 군에 따라 5초, 15초 및 30초 동안
혈관냉동을 시행한 후 혈관경축 해소 효과와 혈관에 미치는 영향에 대해 비교 검토함으로써
다음과 같은 결과를 얻었다.
1. 모든 군에서 혈관냉동후 해빙시 혈관경축은 즉시 해소되었고, 2차 혈관경축 유도시 혈
관 경축은 나타나지 않았다.
2. 해빙 후 동맥의 직경은 대부분 초기 직경보다 0-17% 정도 증가하였으며, 5초군의 동맥
직경은 15초군과 30초군에 비해 다소 작았다(P<0.05).
3. 모든 군에서 혈관냉동후 1일부터 56일째 까지 관찰한 결과 동맥의 직경은 초기 직경의
1-22% 정도 증가되어 있었으며, 각 군간에 유의한 차이는 없었다
4. 모든 군에서 혈관냉동후 56일째 까지 혈관경축 유도시 혈관경축은 나타나지 않았다.
5. 조직학적으로 혈관냉동후 1일째에 혈관벽 내의 세포들은 상실되고, 각 층의 경계가 불
명확하였으며 냉동시간에 따른 혈관 손상의 정도는 거의 차이가 없었으나, 혈관냉동후
시간이 경과함에 따라 혈관냉동 시간이 짧을수록 보다 신속히 치유된 양상을 나타냈다.
6. 냉동후 56일째에 모든 군에서 손상된 혈관은 재생되는 양상을 나타냈고 세포충실도는
증가되었으며, 각 군 간에 차이가 거의 없었다.
이상의 소견으로 미루어 혈관 손상을 최소화하면서 혈관경축을 해소시킬 수 있는 혈관냉
동 시간은 5초라고 생각된다.
#초록#
The outcomes of microvascular surgery may be jeopardized by factors that reduce the
blood flow across the freshly the performed anastomosis Arterial vasospasm is one of
such factors causing the failure of an anastomosis. Vascular spasm has treated by
systemic or topical administration of pharmacologic agents. However, despite the works
done to prevent and elucidate the cause of vasospasm, a apasmolytic agent Capable of
consistently protecting against it or reversing it has not been found. Vascular freezing
as an powerful spasmolytic maneuver has recently been reported.
The purpose of the present study was to determine optimal vascular freezing time.
The effect of vascular freezing time was examined on the relief of arterial vasospasm.
The vascular was freezing was performed on the artery of rats whit hand-held liquid
nitrogen oxide spray. the artery was frozen for 5, 15,or 30 seconds. Vascular spasm and
patency were evaluated immediately after and 1, 3, 7, 14, and 56 days after the freezing.
Femaral arteries were havested at 1, 3, 7, 14, and 56 days after freezing for histologc
examination
The results obtained were as follows :
1. immediately after the freezing-thawing, the vasospasm was relieved and not
induced by spasmogenic induction manipulation in all groups.
2. Immediately after the freezing-thawing, the artery become dialated, the diameter of
which was slightly more than the intial, ranging between 0-17% and the diameter
of 5-second group was smaller than those of 15-and 30-second group(P<0.05).
3. One to 56 days after freezing, arteral diameter was still 1-22% larger than the
initial and there was no significant difference among groups.
4. One to 56 days after the freezing, vasospasm was not induced by spasmogenic
induction manipulation in all groups.
5. One day after the freezing, almost all the vessel wall died and the normal
architeture of the layer was deranged in all group. The shorter was the duration
of freezing, the more early was the healing of artery occurred,
6. Fifty-six days after the freezing, cellularity was increased and the architecture was
regenerated, There was no significant differance among groups.
It was suggested that vascular freezing for 5 seconds is useful to relieve the
vasospasm and to lessen the injury of artery.
키워드
arterial vasospasm; vasospasm relief; vascular freezing;
원문 및 링크아웃 정보
등재저널 정보